effective phages as green antimicrobial agents against antibiotic-resistant hospital escherichia coli
نویسندگان
چکیده
conclusions according to the results, six strains were resistant to six or seven antibiotics and all strains were at least resistant to two antibiotics. however, for each of these resistant bacteria one bacteriophage was isolated from environmental samples, which showed the effectiveness of bacteriophages to remove clinically resistant e. coli strains. effective phages in vitro showed effective results in vivo as well. results ten e. coli strains were isolated from hospital samples. they showed high resistance to the used antibiotics. an effective bacteriophage was isolated for each strain. the cross-reaction showed phages which affect more than six e. coli strains. they can be a good choice for clinical therapeutic use. in animal trials the group challenged with phages after being infected showed similar results as the group treated with gentamicin after being infected. in both groups infection was removed after 48 hours. patients and methods different samples were taken from bed sore and foot ulcers of patients with diabetes. e. coli strains were isolated and identified by standard methods. the antibiogram was ascertained using the kirby bauer disc diffusion method for ten antibiotics. the bacteriophages were isolated from environmental water samples. they were exposed to the host bacteria by the double-layer agar technique (dla) to observe plaques. cross reaction of the phages on test e. coli strains was performed to determine broader-spectrum phages. phage tpr7 was selected for animal trials. five groups of mice including a control group, bacterial group, phage group, antibiotic therapy group and phage therapy group, were examined. background bacteriophages are viruses that attack bacteria and lead to their lysis in an efficient and highly specific manner. these natural enemies of bacteria were used as therapeutic agents before the advent of antibiotics. currently, with the rapid spread of multi-drug resistant bacteria, phage therapy can be an effective alternative treatment for antibiotic resistant bacteria. objectives this study evaluated the effectiveness of bacteriophages in removing antibiotic-resistant clinical escherichia coli strains in vitro and in vivo.
منابع مشابه
Effective Phages as Green Antimicrobial Agents Against Antibiotic-Resistant Hospital Escherichia coli
BACKGROUND Bacteriophages are viruses that attack bacteria and lead to their lysis in an efficient and highly specific manner. These natural enemies of bacteria were used as therapeutic agents before the advent of antibiotics. Currently, with the rapid spread of multi-drug resistant bacteria, phage therapy can be an effective alternative treatment for antibiotic resistant bacteria. OBJECTIVES...
متن کاملGreen tea as an effective antimicrobial for urinary tract infections caused by Escherichia coli
BACKGROUND Urinary tract infections (UTIs) are a very most common type of infection worldwide, and result in billions of dollars in medical care costs. Escherichia coli is the infective agent for 80-90% of all UTIs. Green tea, derived from leaves of the Camellia sinensis plant has been shown to have various potential health benefits (e.g., cardiovascular disease and cancer). The major beneficia...
متن کاملMotuporamine Derivatives as Antimicrobial Agents and Antibiotic Enhancers against Resistant Gram‐Negative Bacteria
Dihydromotuporamine C and its derivatives were evaluated for their in vitro antimicrobial activities and antibiotic enhancement properties against Gram-negative bacteria and clinical isolates. The mechanism of action of one of these derivatives, MOTU-N44, was investigated against Enterobacter aerogenes by using fluorescent dyes to evaluate outer-membrane depolarization and permeabilization. Its...
متن کاملToward Repurposing Ciclopirox as an Antibiotic against Drug-Resistant Acinetobacter baumannii, Escherichia coli, and Klebsiella pneumoniae
Antibiotic-resistant infections caused by gram-negative bacteria are a major healthcare concern. Repurposing drugs circumvents the time and money limitations associated with developing new antimicrobial agents needed to combat these antibiotic-resistant infections. Here we identified the off-patent antifungal agent, ciclopirox, as a candidate to repurpose for antibiotic use. To test the efficac...
متن کاملIdentification of Cyclic Dipeptides from Escherichia coli as New Antimicrobial Agents against Ralstonia Solanacearum.
Ralstonia solanacearum is a causative agent of bacterial wilt in many important crops throughout the world. How to control bacterial wilt caused by R. solanacearum is a major problem in agriculture. In this study, we aim to isolate the biocontrol agents that have high efficacy in the control of bacterial wilt. Three new bacterial strains with high antimicrobial activity against R. solanacearum ...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
jundishapur journal of microbiologyجلد ۸، شماره ۲، صفحات ۰-۰
کلمات کلیدی
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023